Adenosine antagonists potentiate D2 dopamine-dependent activation of Fos in the striatopallidal pathway

• Published in: Neuroscience Vol. 68; no. 3; pp. 721 - 728
• Main Authors: POLLACK, A. E, FINK, J. S
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier 01-10-1995
• Subjects: Adenosine - antagonists & inhibitors; Adrenergic Uptake Inhibitors - pharmacology; Animals; Biochemistry and metabolism; Biological and medical sciences; Caffeine - analogs & derivatives; Caffeine - pharmacology; Central nervous system; Dopamine Agonists - pharmacology; Ergolines - pharmacology; Fundamental and applied biological sciences. Psychology; Genes, fos - drug effects; Globus Pallidus - drug effects; Globus Pallidus - metabolism; Immunohistochemistry; Male; Neostriatum - drug effects; Neostriatum - metabolism; Neural Pathways - drug effects; Neural Pathways - metabolism; Phosphodiesterase Inhibitors - pharmacology; Quinpirole; Rats; Rats, Sprague-Dawley; Receptors, Dopamine D2 - agonists; Reserpine - pharmacology; Theobromine - analogs & derivatives; Theobromine - pharmacology; Theophylline - pharmacology; Vertebrates: nervous system and sense organs; Rat; Rodentia; Central nervous system; Basal ganglion; Corpus striatum; Gene expression; Pallidum; Vertebrata; Mammalia; D2 Dopamine receptor; Antagonist; Adenosine receptor; Brain (vertebrata); Immediate early gene
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/0306-4522(95)00168-I

Adenosine antagonists potentiate dopamine-mediated behaviours. A2a adenosine and D2 dopamine receptors are abundantly co-expressed within the striatopallidal subset of striatal neurons, suggesting that this is the site of interaction between A2a and D2 receptors. We show that the D2-dependent induction of the immediate early gene c-Fos occurs in striatopallidal neurons 3 h following injection of reserpine (10 mg/kg). We used this paradigm to test whether adenosine antagonists modulate D2-dependent activation of striatopallidal neurons. The non-selective A1-A2 adenosine antagonists theophylline (25 mg/kg) or 3,7-dimethyl-1-propargylxanthine (25 mg/kg) potentiated the effect of a submaximal dose of the D2 dopamine agonist quinpirole (0.05 mg/kg) to prevent the induction of striatal c-Fos following reserpine. Co-administration of the A2a receptor antagonist 8-(3-chlorostyryl) caffeine (5 mg/kg) with quinpirole (0.05 mg/kg) also attenuated striatal c-Fos induction following reserpine to a greater extent than 0.05 mg/kg quinpirole alone. When administered prior to reserpine, theophylline (25 mg/kg) or 3,7-dimethyl-1-propargylxanthine (25 mg/kg) partially attenuate the induction of striatal c-fos. These results demonstrate that systemic administration of adenosine antagonists enhance D2 dopamine receptor-dependent regulation of c-Fos in the striatopallidal pathway. These results support a functional interaction between A2a adenosine and D2 dopamine receptors in striatopallidal neurons.