Nanoparticle-containing lyophilized dry powder inhaler formulations optimized using central composite design with improved aerodynamic parameters and redispersibility

• Published in: Pharmaceutical development and technology Vol. 28; no. 1; pp. 124 - 137
• Main Author: Akdag, Yagmur
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02-01-2023
• Subjects: A549 Cells; Administration, Inhalation; Aerosols; Dry powder inhaler; Dry Powder Inhalers; human serum albumin; Humans; l-leucine; lyophilization; mannitol; Nanoparticles - chemistry; paclitaxel; Particle Size; Powders; Dry powder inhaler; paclitaxel; lyophilization; l-leucine; human serum albumin; mannitol
• ISSN: 1083-7450; 1097-9867
• DOI: 10.1080/10837450.2023.2166066

Objectives: The aim of this study was to improve the aerodynamic behavior and redispersibility of a lyophilized dry powder inhaler (DPI) formulation containing nanoparticles.
Methods: Paclitaxel (PTX)-human serum albumin (HSA) nanoparticles were used as a model, and DPIs containing the nanoparticles were produced by lyophilization using different carriers and carrier ratios. A central composite design was employed to optimize the formulation. L-leucine and mannitol were chosen as independent variables, and mass median aerodynamic diameter (MMAD), emitted fraction, fine particle fraction (FPF), nanoparticle size, polydispersity index (PDI), zeta potential were selected as dependent variables.
Results: The water content of DPIs was less than 5% for all DPIs. The cytotoxicity of the DPIs, determined using A549 cells, was due to PTX alone. Particle sizes of 204.3 ± 1.65 nm and 94.3-1353.0 nm were obtained before and after lyophilization, respectively. The developed method resulted in a reduction in the MMAD from 8.148 µm to 5.274 µm, an increase in the FPF from 17.63% to 33.60%, and an increase in the emitted fraction from 77.68% to 97.03%. The physico-chemical characteristics of the optimized formulation were also assessed.
Conclusions: In conclusion, this study demonstrates that lyophilization can be used to produce nanoparticle-containing DPI formulations with improved redispersibility and aerodynamic properties.