Sludge and lignin as growth media for the production of antimicrobial substances by selected microorganisms

• Published in: Archiv für Phytopathologie und Pflanzenschutz Vol. 42; no. 8; pp. 783 - 795
• Main Authors: Mandryk, Maryna N., Kolomiets, Emily I., Shapaval, Volha, Dey, Estera S.
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 01-08-2009
• Subjects: antimicrobial substance; Bacillus; Beauveria; Botrytis cinerea; digested sludge; enzyme-pre-digested sludge; Feed sludge; Fusarium oxysporum; humic acid; lignin; Paecilomyces; Pseudomonas aurantiaca; Pseudomonas syringae; Streptomyces; Trichoderma; Trichosporon
• ISSN: 0323-5408; 1477-2906
• DOI: 10.1080/03235400701453253

The ability of sludge, lignin and humic acid to support the growth and production of antimicrobial substances by 19 microorganism strains was tested. Organisms from seven genera: Bacillus, Pseudomonas, Streptomyces, Beauveria, Paecilomyces, Trichoderma and Trichosporon grew on the tested media. On these media, they were also examined for the production of anti-phytopathogenic activity against Fusarium oxysporum P1 BIM, Botrytis cinerea B1 BIM and Pseudomonas syringae. pv. glicinea B-280 BIM. Strains Pseudomonas aurantiaca S-1, Bacillus sp. 19 and Streptomyces sp. 11 synthesize antimicrobial metabolites on the different media. Protease, cellulase, α-amylase, xylanase and peroxidase were also produced by the same microorganisms, which can explain their ability to grow on sludge, and laccase was produced only by P. aurantiaca S-1. HPLC analysis of the lignin culture media before and after P. aurantiaca S-1 growth has shown that the major lignin peak in the culture broth disappeared.