Randia ferox (Cham & Schltdl) DC.: phytochemical composition, in vitro cyto- and genotoxicity analyses

• Published in: Natural product research Vol. 36; no. 16; pp. 4170 - 4176
• Main Authors: Pappis, Lauren, Ramos, Andiara Prates, Fontana, Tuyla, Sangoi, Gabriela Geraldo, Dornelles, Rafaela Castro, Dolwitsch, Carolina Bolssoni, Sagrillo, Michele Rorato, Cadoná, Francine Carla, Machado, Alencar Kolinski, Bauermann, Liliane de Freitas
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis Ltd 01-08-2022
• Subjects: Biocompatibility; Cell proliferation; Cell viability; Chemical composition; Damage; DNA damage; Erythrocytes; Fibroblasts; Genotoxicity; Hemolysis; Indigenous species; Leukocytes (mononuclear); Macrophages; Medicinal plants; Nitric oxide; Randia; Reactive oxygen species; Safety; Toxicology
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2021.1960522

Randia ferox is a Brazilian native species used in folk medicine. Scientific information regarding the toxicology and phytochemistry of this plant remains unclear. We aimed to produce a R. ferox extract, identify its chemical matrix, and evaluate its safety profile. The extract chemical composition was accessed through UHPLC-MS/MS. Mononuclear cells, erythrocytes, fibroblasts, macrophages, and kidney cells were subjected to extract concentration-response curve testing. The cellular viability, proliferation, dsDNA release, reactive oxygen species (ROS), nitric oxide (NO), hemolysis, and DNA damage were determined. Ten molecules were found in the extract matrix. Most of the tested concentrations can be considered safe. Cellular viability, proliferation, dsDNA release, and NO remained at similar levels to the control. The extract increased ROS in macrophages. None of the tested concentrations induced DNA damage or hemolysis. The data suggest R. ferox extract contains several bioactive molecules and has a safety profile in vitro.