Innovative synthesis and molecular modeling of actinomycetes-derived silver nanoparticles for biomedical applications

• Published in: Microbial pathogenesis Vol. 196; p. 106990
• Main Authors: Ahmed Amin, Safia, Dawood, Mohamed E.A., Mahmoud, Mohamed, Bassiouny, Dina M., Moustafa, Mahmoud M.A., Abd El Ghany, Khalid
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-11-2024
• Subjects: Actinomycetes; Antibacterial activity; Ion ligand prediction and modeling; Molecular characterization; Nanoparticles; Wound healing; Nanoparticles; Antibacterial activity; Actinomycetes; Wound healing; Molecular characterization; Ion ligand prediction and modeling
• ISSN: 0882-4010; 1096-1208; 1096-1208
• DOI: 10.1016/j.micpath.2024.106990

The rising demand for innovative antimicrobial solutions has shifted focus towards silver nanoparticles (AgNPs), especially those produced through eco-friendly methods. This study introduces a novel approach utilizing actinomycetes strains—Streptomyces albus, Micromonospora maris, and Arthrobacter crystallopoietes—to biosynthesize AgNPs with remarkable antibacterial properties. Through molecular characterization, we identified unique features of these nanoparticles, and computational modeling suggested significant ion-ligand interactions with proteins 6REV and 3K07. Our research highlights the promise of these biogenically synthesized nanoparticles in advancing biomedical applications. Actinomycetes were sourced and screened for their ability to produce metallic nanoparticles, revealing that among 35 samples, only six showed this capability. Notably, Streptomyces albus strain smmdk14 (OR685674), Micromonospora maris strain smmdk13 (OR685672), and Arthrobacter crystallopoietes strain smmdk12 (OR685674) were identified as effective silver nanoparticle producers. The synthesized nanoparticles demonstrated potent antibacterial activity against common pathogens including E. coli, Pseudomonas aeruginosa, Klebsiella spp., Enterococcus faecalis, Staphylococcus aureus, and Acinetobacter spp. The data obtained from color change observation, UV–visible spectrophotometry, Zeta potential, FTIR spectroscopy, and transmission electron microscopy (TEM) characterized AgNPs potentiality. The nanoparticles were spherical, with sizes ranging from 6.46 nm to 24.7 nm. Optimization of production conditions, comparison of antimicrobial effects with antibiotics, evaluation of potential toxicity, and assessment of wound-healing capabilities were also conducted. The biosynthesized AgNPs exhibited superior antibacterial properties compared to traditional antibiotics and significantly accelerated wound healing by approximately 66.4 % in fibroblast cell cultures. Additionally, computational analysis predicted interactions between various metal ions and specific amino acid residues in proteins 6REV and 3K07. Overall, this study demonstrates the successful creation of AgNPs with notable antibacterial and wound-healing properties, underscoring their potential for medical applications.
•Innovative Antimicrobial Solutions: Silver nanoparticles (AgNPs) produced using eco-friendly methods are gaining attention for their antibacterial properties.•Enhanced Biomedical Potential: Compared to traditional antibiotics, the silver nanoparticles displayed superior antibacterial properties and accelerated wound healing by 66.4 % in fibroblast cultures.•Eco-Friendly and Effective: The study underscores the promise of biogenically synthesized AgNPs for advancing medical applications, from combating infections to promoting wound healing.