Organic arabic coffee husk: Antioxidant and cytoprotective properties and potential impacts on selected human intestinal bacterial populations of individuals with diabetes

• Published in: Food research international Vol. 192; p. 114730
• Main Authors: Abreu, Thaianaly Leite, Silva, Gezaildo Santos, de Farias Marques, Antonia Dayane Jenyffer, de Espindola, Juliana Santos, de Assis, Bianca Beatriz Torres, Sampaio, Karoliny Brito, de Albuquerque, Thatyane Mariano Rodrigues, Pacheco, Maria Teresa Bertoldo, Galland, Fabiana Andrea Barrera, de Souza, Evandro Leite, Magnani, Marciane, Madruga, Marta Suely, Bezerra, Taliana Kênia Alencar
• Format: Journal Article
• Language: English
• Published: Canada Elsevier Ltd 01-09-2024
• Subjects: Antioxidant activity; Antioxidants - pharmacology; Bacteria - drug effects; By-products; Caco-2 Cells; Coffea - chemistry; Coffee - chemistry; Diabetes Mellitus; Fermentation; Gastrointestinal Microbiome - drug effects; Gut microbiota; Humans; Organic coffee; Phenolic compounds; Gut microbiota; CHE; DMSO; H2O2; MTT; AUC; DMEM; FBS; FRAP; HPLC; ORAC; PBS; Organic coffee; DPPH; By-products; CH; Antioxidant activity; DCF; Phenolic compounds; EDTA; AnaeroGen; ROO; UV–VIS; TEAC; RAC; NC; ROS; APPH; DCF-DA
• ISSN: 0963-9969; 1873-7145; 1873-7145
• DOI: 10.1016/j.foodres.2024.114730

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•Organic coffee husks demonstrated considerable post-digestion antioxidant potential in vitro.•Coffee husks enhance cell viability, inhibit ROS.•Caco-2 cell oxidation was attenuated upon exposure to organic coffee husk extract.•In vitro colonic fermentation of coffee husks positively impacts microbiota in diabetes.•Organic Coffee Husk presence positively correlated with phenolic release.
Coffee husks are the main by-product of the coffee industry and have been traditionally discarded in the environment or used as fertilizers. However, recent studies have shown that coffee husks have bioactive compounds, such as phenolics and fiber-bound macro antioxidants, offering a range of potential health benefits. This study evaluated the antioxidant capacity, cytoprotective/cytotoxic properties, and stimulatory effects on the relative abundance of selected intestinal bacterial populations of individuals with diabetes of organic coffee husks. Organic coffee husk had good antioxidant capacity, maintained under simulated gastric conditions, with more than 50% of antioxidant capacity remaining. Organic coffee husk exerted cytoprotective properties in Caco-2 cells, indicating that cellular functions were not disturbed, besides not inducing oxidation. Overall, organic coffee husk promoted positive effects on the abundance of distinct intestinal bacterial groups of individuals with diabetes during in vitro colonic fermentation, with a higher relative abundance of Bifidobacterium spp., indicating the availability of components able to reach the colon to be fermented by intestinal microbiota. Organic coffee husk could be a circular material to develop new safe and pesticide-free functional ingredients with antioxidant and potential beneficial effects on human intestinal microbiota.