Phylogenetic study of Theileria lestoquardi based on 18SrRNA gene Isolated from sheep in the middle region of Iraq

• Published in: Iraqi journal of veterinary sciences Vol. 30; no. 2; pp. 27 - 32
• Main Authors: al-Khalidi, Mansur Jadan Ali, Ayiz, Numan Naji, Nasir, Hasan Hajim
• Format: Journal Article
• Language: Arabic; English
• Published: Mosul, Iraq University of Mosul, College of Veterinary Medicine 2016
• Subjects: 18S rRNA gen; Ovine; PCR; Phylogenetic Analysis; Theileria; الأغنام; دراسات الحالة; علم تطور السلالات
• ISSN: 1607-3894; 2071-1255; 2071-1255
• DOI: 10.33899/ijvs.2016.121380

Theileriosis is parasitic infection causes by obligate intracellular protozoa of the genus Theileria. T. lestoquardi is the most
virulent species in sheep and goats which causes a severe disease with a high morbidity and mortality rate. In this study the
phylogenetic relationships between two local isolate of T. lestoquardi and nine T. lestoquardi global isolates as well as
Babesia ovis out-group isolate were analyzed using the 18S rRNA gene sequence. The multiple sequence alignment analysis
and neighbor joining phylogenetic tree analysis were performed by using ClustalW multiple sequence alignment online based
analysis of 1098bp 18S rRNA gene was amplified by polymerase chain reaction. Phylogenetic analysis results of these gene
sequences revealed that T. lestoquardi local isolates were closely related to T. lestoquardi Iran isolate (JQ917458.1) and two
Iraq Kurdistan isolates (KC778786.1 and KC778785.1) more than other countries. This study represents the first report on the
use of molecular phylogeny to classify T. lestoquardi obtained in Middle Region of Iraq.