Enzymic activities and gene expression of enzymes of the acyl-CoA elongase during rapeseed development

• Published in: Biochemical Society transactions Vol. 28; no. 6; p. 645
• Main Authors: Puyaubert, J, Garbay, B, Dieryck, W, Costaglioli, P, Roscoe, T, Cassagne, C, Lessire, R
• Format: Journal Article
• Language: English
• Published: England 01-12-2000
• Subjects: Acyl-Carrier Protein S-Malonyltransferase; Acyltransferases - genetics; Acyltransferases - metabolism; Brassica - enzymology; Brassica - genetics; Brassica - growth & development; Gene Expression Regulation, Developmental; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Reverse Transcriptase Polymerase Chain Reaction; Seeds - enzymology
• ISSN: 0300-5127
• DOI: 10.1042/bst0280645

Enzymic activities and gene expression of oleoyl-CoA elongase were studied during seed development using two different rapeseed cultivars, high-erucic-acid rapeseed (HEAR) and low-erucic-acid rapeseed (LEAR). The overall elongase activities were maximal in HEAR between the fourth and eighth weeks after pollination (WAP) and absent in LEAR. The 3-ketoacyl-CoA synthase (condensing enzyme, CE) mRNA levels and the developmental profiles in the two cultivars were different since maximal expression levels were detected in HEAR and LEAR at WAP 4 and WAP 6, respectively. Anti-CE antibodies revealed two proteins of 60 and 67 kDa in both cultivars and an additional reacting protein of 57 kDa in HEAR.