Cytogenetic, histopathologic, and immunologic studies of multifocal renal cell carcinoma

• Published in: Cancer Vol. 79; no. 5; pp. 975 - 981
• Main Authors: Junker, Kerstin, Schlichter, Andreas, Junker, Udo, Knöfel, Brigitte, Kosmehl, Hartwig, Schubert, Jörg, Claussen, Uwe
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01-03-1997; Wiley-Liss
• Subjects: Biological and medical sciences; Carcinoma, Renal Cell - immunology; Carcinoma, Renal Cell - pathology; Carcinoma, Renal Cell - secondary; Chromosome Aberrations - pathology; Chromosome Banding; Chromosome Disorders; chromosomes; cytogenetics; Female; Humans; immunology; In Situ Hybridization, Fluorescence; In Vitro Techniques; Karyotyping; Kidney; Kidney Neoplasms - immunology; Kidney Neoplasms - pathology; Kidney Neoplasms - secondary; Kidneys; Medical sciences; multifocal; Neoplasm Metastasis; Nephrology. Urinary tract diseases; renal cell carcinoma; Tumors of the urinary system; Chromosomal aberration; Kidney disease; Human; Molecular hybridization; Urinary system disease; Carcinoma; Transforming growth factor β; In situ; Cytokine; Interleukin 11; Malignant tumor; Potential; Interleukin 6; Pathology; Histopathology; Immunological investigation; Interleukin 10; Cytogenetics; Genetics; Abnormal chromosome; Grawitz tumor; Metastatic; Molecular biology; Karyotype
• ISSN: 0008-543X; 1097-0142
• DOI: 10.1002/(SICI)1097-0142(19970301)79:5<975::AID-CNCR14>3.0.CO;2-#

BACKGROUND
Multifocal tumor areas occurred in 12‐22% of patients with renal cell carcinoma. It is unknown whether these tumors have malignant potential and characterize a higher risk for metastases. The performance of nephron‐sparing surgery in patients with low grade or low stage tumors is controversial.
METHODS
Primary and secondary tumors were analyzed by conventional cytogenetics and fluorescence in situ hybridization. Production of interleukin (IL)‐6, IL‐10, IL‐11, and transforming growth factor (TGF)‐β1 were determined using standard enzyme‐linked immunosorbent assay and bioassays.
RESULTS
In 15.2% of the renal cell carcinoma cases evaluated, multifocal tumors were detected. Cytogenetics revealed a concordance of primary and secondary tumors in 9 of 14 cases (64%). In 11 of 12 multifocal tumors (94%), the same immunologic activity status was observed in both primary and secondary tumors.
CONCLUSIONS
Secondary tumors must be expected to have malignant potential similar to that of the primary tumors. This was underscored by the high concordance of cytogenetic, histopathologic, and immunologic data in this study. Cancer 1997; 79:975‐81. © 1997 American Cancer Society.
In this study, cytogenetic, immunologic, and histopathologic data showed that secondary multifocal renal cell carcinomas must be expected to have malignant potential similar to that of the primary tumors.