Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS

• Published in: Journal of Zhejiang University. B. Science Vol. 6; no. 1; pp. 74 - 78
• Main Author: 潘春秀 徐秀珠 何红梅 蔡小军 张雪君
• Format: Journal Article
• Language: English
• Published: China Department of Chemistry, Zhejiang University, Hangzhou 310027, China 01-01-2005; Zhejiang University Press
• Subjects: 2-丁烯-1,4-二醇; Acetamides - analysis; Acetamides - chemistry; Butylene Glycols - analysis; Butylene Glycols - chemistry; Chemical Fractionation - methods; Chemistry; Chromatography, High Pressure Liquid - methods; HPLC; Isomerism; lafutidine; LC-MS; Mass Spectrometry - methods; Piperidines - analysis; Piperidines - chemistry; Pyridines - analysis; Pyridines - chemistry; 分子识别; 反式异构体; 顺式异构体; 高效液相色谱法; 2-butene-1,4-diol,Lafutidine,Isomers separation,(S,S)-Whelk-O 1,ChiraSpher,LC-MS
• ISSN: 1673-1581; 1862-1783
• DOI: 10.1631/jzus.2005.B0074

The cis and trans isomers separation of2-butene- 1,4-diol and lafutidine were studied by HPLC on two kinds of chiral columns: (S,S)-Whelk-O 1 and ChiraSpher. The isomers of 2-butene-1,4-diol can be separated on both chiral columns while the isomers of lafutidine can only be resolved on ChiraSpher column. The influence of different type and amount of mobile phase modifier on the isomers separation was extensively studied. The resolution of cis and trans isomers of 2-butene-1,4-diol was 2.61 on (S,S)-Whelk-O 1 column with hexane-ethanol (97:3, v/v) as the mobile phase. The resolution of lafutidine was 1.89 on ChiraSpher column with hexane-ethanol-THF-diethylamine (92:3:5:0.1, v/v/v/v) as the mobile phase. LC-MS methods were developed to identify the isomer peaks.