Iamia majanohamensis gen. nov., sp. nov., an actinobacterium isolated from sea cucumber Holothuria edulis, and proposal of Iamiaceae fam. nov

• Published in: International journal of systematic and evolutionary microbiology Vol. 59; no. 4; pp. 869 - 873
• Main Authors: Kurahashi, Midori, Fukunaga, Yukiyo, Sakiyama, Yayoi, Harayama, Shigeaki, Yokota, Akira
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01-04-2009
• Subjects: Acidimicrobium ferrooxidans; Actinobacteria; Actinobacteria - chemistry; Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Animals; Bacterial Typing Techniques; Base Composition; Carbohydrates - analysis; Cell Wall - chemistry; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Holothuria - microbiology; Holothuria edulis; Holothurioidea; Iamia majanohamensis; Japan; Marine; Molecular Sequence Data; Peptidoglycan - analysis; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Vitamin K 2 - analysis; Japan; INW, Japan
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.005611-0

1 Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-0032, Japan
2 NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusa-kamatari, Kisarazu-shi, Chiba 292-0818, Japan
Correspondence Midori Kurahashi kuramido{at}iam.u-tokyo.ac.jp
A novel, Gram-positive bacterial strain, F12 T , was isolated from the abdominal epidermis of a sea cucumber, Holothuria edulis , collected from seawater off the coast of Japan. According to 16S rRNA gene sequence analysis, this strain represents a novel, deep-rooting lineage within the class Actinobacteria and clusters with uncultured bacteria and Acidimicrobium ferrooxidans . Compared to species with validly published names, the highest 16S rRNA gene sequence similarity (89.8 %) was to Acidimicrobium ferrooxidans DSM 10331 T . Phylogenetic analyses showed that strain F12 T represents a distinct phylogenetic lineage related closely to the genus Acidimicrobium . Strain F12 T contained MK-9(H 6 ) as the major menaquinone, whilst 17 : 0, 17 : 1 8 c , 15 : 0 and 16 : 0 were the major cellular fatty acids. The cell-wall peptidoglycan of strain F12 T was composed of meso -diaminopimelic acid as the diagnostic diamino acid, alanine and glutamic acid (1 : 2 : 1). The cell-wall sugars detected were rhamnose, mannose, arabinose, galactose and xylose. The G+C content of the DNA was 74.4 mol%. From the taxonomic data obtained in this study, the name Iamia majanohamensis gen. nov., sp. nov. is proposed for the isolate, with type strain F12 T (=NBRC 102561 T =DSM 19957 T ). The name Iamiaceae fam. nov. is also proposed for the distinct phyletic line represented by the genus Iamia .
Abbreviations: DAP, diaminopimelic acid; ML, maximum likelihood; NJ, neighbour joining; TEM, transmission electron microscopy
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain F12 T is AB360448 .
A supplementary figure showing the general morphology of cells of strain F12 T is available with the online version of this paper.