Polynucleotide Sequence Analysis by Sequential Base Elimination: 3′-Terminus of Phage Qβ RNA

• Published in: Science (American Association for the Advancement of Science) Vol. 166; no. 3908; pp. 1004 - 1005
• Main Authors: Weith, H. L., Gilham, P. T.
• Format: Journal Article
• Language: English
• Published: United States American Association for the Advancement of Science 21-11-1969
• Subjects: Alkalies; Bacteriophages; Bacteriophages - analysis; Chemical bases; Chromatography, Ion Exchange; Edetic Acid; Hemoglobins; Kinetics; Methods; Nucleosides; Nucleotides - analysis; Oligonucleotides; Oxidation-Reduction; Polynucleotides; Polynucleotides - analysis; Ribonucleases - metabolism; RNA; RNA, Viral - analysis; Sequence analysis
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.166.3908.1004

The nucleotide sequence of a 3′-terminal fragment obtained by ribonuclease T$_{1}$ hydrolysis of the ribonucleic acid from bacteriophage Qβ has been determined by an improved method of sequence analysis which involves sequential removal of bases by periodate oxidation and beta-elimination. The results obtained from ten such oxidation-elimination cycles and from the alkaline hydrolysis of the remaining oligonucleotide indicate that the first 16 nucleotides at the 3′-terminus of this ribonucleic acid have the sequence: -G-C-C-C-U-C-U-C-U-C-C-U-C-C-C-A.