A near-infrared fluorescent probe for ratiometric imaging peroxynitrite in Parkinson's disease model

• Published in: Sensors and actuators. B, Chemical Vol. 359; p. 131393
• Main Authors: Kang, Hao, Shu, Wei, Yu, Jin, Gao, Mengxu, Han, Rubing, Jing, Jing, Zhang, Rubo, Zhang, Xiaoling
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15-05-2022; Elsevier Science Ltd
• Subjects: Fluorescent indicators; Fluorescent probe; Hydrogen sulfide; Infrared tracking; Medical imaging; Near-infrared; Parkinson's disease; Pathogenesis; Peroxynitrite; Ratiometric; Selectivity; Zebrafish; Peroxynitrite; Parkinson’s disease; Fluorescent probe; Ratiometric; Near-infrared
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2022.131393

As a regular neurodegenerative disease, Parkinson's disease (PD) brings great pain and heavy economic burden to patients. Peroxynitrite (ONOO-) have attracted great attention to be a neurotoxicity specie in the pathogenesis of PD. Therefore, understanding the physiological functions of ONOO- in PD disease is of great importance to the early diagnoses. Unfortunately, it still lacks effective method for detecting ONOO- in PD model. In this work, a highly sensitivity and selectivity near-infrared ratiometric fluorescent probe (named K-ONOO) was designed for tracking ONOO- in PD model. K-ONOO exhibited a unique ratiometric response toward ONOO- due to the fracture of the boronic acid ester group and the principle of ICT resulted in red-shifted spectra. K-ONOO exhibited a quantitative response to ONOO- (0–15 μM) with a low detection limit (212 nM). K-ONOO can successfully map the changes of endogenous ONOO- in vivo. The results demonstrated that an elevated degree of ONOO- is closely correlated with zebrafish under rotenone stimulation. More importantly, H2S may serve as a neuroprotectant, which helps regulate ONOO- overexpression and prevent oxidative stress-induced neurodegeneration. The visualization imaging of ONOO- based on K-ONOO provides an auspicious method for understanding the essential role of ONOO- during PD disease pathology and early diagnosis.
•A NIR fluorescent probe K-ONOO with ratiometric characteristic for imaging ONOO- in PD model was reported.•K-ONOO is the first NIR ratiometric fluorescent probe for imaging ONOO- in PD model.•K-ONOO displayed high specificity toward ONOO- over other reactive oxygen species with a fast response.•K-ONOO was successfully applied for monitoring the fluctuation of ONOO- in PD model.•H2S may serve as a latent neuroprotectant to prevent neurotoxin-induced neurodegeneration.