Protamine 1 and 2 mRNA Abundance in Human Spermatozoa and Its Relation to Semen Quality and Sperm DNA Fragmentation among Fertility Clinic Patients

• Published in: Russian journal of genetics Vol. 57; no. 2; pp. 213 - 220
• Main Authors: Ishchuk, M. A., Malysheva, O. V., Komarova, E. M., Mekina, I. D., Lesik, E. A., Gzgzyan, A. M., Kogan, I. Yu, Baranov, V. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-02-2021
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; Human Genetics; Microbial Genetics and Genomics; human sperm; sperm DNA fragmentation; male infertility; sperm RNA; assisted reproductive technologies
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795421020058

Sperm contain a complex population of coding and noncoding RNA, and the utility of sperm RNA in fertility research is currently being explored. The aim of this study was to estimate the content of transcripts of protamines 1 and 2 in human ejaculated spermatozoa in relation to semen quality and sperm DNA fragmentation between fertile and infertile patients. Human ejaculates were obtained from 33 patients and semen analyzes were assessed by WHO criteria (2010). We evaluated the sperm DNA fragmentation measured by TUNEL assay. The ejaculates of patients were purified by density-gradient centrifugation, sperm cells were lysed, and mRNA was extracted, reverse transcribed, and subjected to real-time qPCR using specific primer pairs for protamine-1, protamine-2 RNA. The sperm protamine mRNA ratio in normozoospermic men (
n
 = 19; 2.86 ± 0.16) differed significantly from that of patozoospermic patients (
n
= 14; 3.43 ± 0.22;
p
< 0.05). A significant correlation was shown between sperm DNA fragmentation and the
PRM2
/
PRM1
mRNA ratio (
r
= 0.33;
p
< 0.05). In the group of patients with an increased sperm DNA fragmentation (
n
= 14; 2.74 ± 0.18), the
PRM2
/
PRM1
ratio was significantly higher than in the group of patients with normal rates (
n
= 19; 3.26 ± 0.19;
p
< 0.05). An abnormal sperm protamine ratio was associated with poor semen quality and DNA fragmentation. Finding sperm-quality markers would help to understand the causes of male infertility and to improve male reproductive health.