Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk
The objective of this study was to detect tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) and tanker trailer's (n = 113) samples, two methods were used simultaneously as follows: a specific rapid test Milk Tetrasensor Kit an...
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Published in: | Czech Journal of Food Sciences Vol. 27; no. 5; pp. 379 - 385 |
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Main Authors: | , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Prague
Czech Academy of Agricultural Sciences (CAAS)
31-10-2009
Czech Academy of Agricultural Sciences |
Subjects: | |
Online Access: | Get full text |
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Summary: | The objective of this study was to detect tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) and tanker trailer's (n = 113) samples, two methods were used simultaneously as follows: a specific rapid test Milk Tetrasensor Kit and HPLC with ultraviolet detection and isocratic elution. The samples underwent solid phase extraction before the HPLC analysis. The Nova Pack C8 column (3.9 x 150 mm, 4 microm) and mobile phase (0.8 mL/min) consisting of acetonitrile, methanol, and 0.05 mol/L of oxalic acid in a 13:13:74 ratio were used. None of the samples analysed with the use of the specific rapid test displayed the presence of tetracycline antibiotics. In all of the samples analysed by means of HPLC, low concentrations of tetracycline antibiotics residues were detected. None of the samples displayed the presence of chlortetracycline. All the analysed samples displayed residues of tetracycline. Oxytetracycline residues were detected only in 50.6% of analysed samples. |
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Bibliography: | http://www.agriculturejournals.cz/uniqueFiles/11698.pdf 2010000024 L70 Q03 |
ISSN: | 1212-1800 1805-9317 |
DOI: | 10.17221/177/2008-CJFS |