Occurrence of tetracycline, chlortetracycline, and oxytetracycline residues in raw cow's milk

The objective of this study was to detect tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) and tanker trailer's (n = 113) samples, two methods were used simultaneously as follows: a specific rapid test Milk Tetrasensor Kit an...

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Published in:Czech Journal of Food Sciences Vol. 27; no. 5; pp. 379 - 385
Main Authors: Navratilova, P.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Mleka, Borkovcova, I.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Mleka, Drackova, M.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Mleka, Janstova, B.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Mleka, Vorlova, L.,Veterinarni a Farmaceuticka Univ., Brno (Czech Republic). Ustav Hygieny a Technologie Mleka
Format: Journal Article
Language:English
Published: Prague Czech Academy of Agricultural Sciences (CAAS) 31-10-2009
Czech Academy of Agricultural Sciences
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Summary:The objective of this study was to detect tetracycline, chlortetracycline and oxytetracycline residues in raw cow's milk. When analysing bulk milk (n = 57) and tanker trailer's (n = 113) samples, two methods were used simultaneously as follows: a specific rapid test Milk Tetrasensor Kit and HPLC with ultraviolet detection and isocratic elution. The samples underwent solid phase extraction before the HPLC analysis. The Nova Pack C8 column (3.9 x 150 mm, 4 microm) and mobile phase (0.8 mL/min) consisting of acetonitrile, methanol, and 0.05 mol/L of oxalic acid in a 13:13:74 ratio were used. None of the samples analysed with the use of the specific rapid test displayed the presence of tetracycline antibiotics. In all of the samples analysed by means of HPLC, low concentrations of tetracycline antibiotics residues were detected. None of the samples displayed the presence of chlortetracycline. All the analysed samples displayed residues of tetracycline. Oxytetracycline residues were detected only in 50.6% of analysed samples.
Bibliography:http://www.agriculturejournals.cz/uniqueFiles/11698.pdf
2010000024
L70
Q03
ISSN:1212-1800
1805-9317
DOI:10.17221/177/2008-CJFS