Effects of exercise training with Lactobacillus plantarum intake on ionized calcium‐binding adaptor molecule 1, glial fibrillary acidic protein, interleukin‐6 and interleukin‐1β in cuprizone‐induced demyelination mouse model of multiple sclerosis

• Published in: Clinical & experimental neuroimmunology Vol. 13; no. 1; pp. 42 - 53
• Main Authors: Sajedi, Donya, Shabani, Ramin, Elmieh, Alireza
• Format: Journal Article
• Language: English
• Published: Ube Wiley Subscription Services, Inc 01-02-2022
• Subjects: Calcium; Central nervous system; Cuprizone; Cytokines; Demyelination; endurance training; Exercise; Fitness training programs; Gene expression; Glial fibrillary acidic protein; interleukins; Lactobacillus plantarum; Multiple sclerosis; Neurodegeneration; Physical training; Probiotics; Proteins
• ISSN: 1759-1961; 1759-1961
• DOI: 10.1111/cen3.12673

Objective
Multiple sclerosis (MS) is a central nervous system disorder characterized by inflammation, demyelination and neurodegeneration. It has been suggested that exercise and intake of probiotics might influence MS pathology and, thereby, slow down the disease process in MS patients. Therefore, this study aimed to investigate the effect of exercise training with Lactobacillus plantarum intake on ionized calcium‐binding adaptor molecule 1, glial fibrillary acidic protein, interleukin (IL)‐6 and IL‐1β in cuprizone‐induced demyelination mouse model of MS.
Methods
Mice were exposed to cuprizone for 13 weeks. In the fifth week, motor and balance tests were carried out on them. The mice (n = 5 per group) were randomly divided into five groups: control (C), MS, MS with exercise (MS + Exe), MS with probiotics (MS + Pro) and MS with probiotics and exercise (MS + Pro + Exe). The exercise groups carried out aerobic exercises 5 days a week for 2 months. The mice received probiotic by gavage. One day after the interventions finished, the mice were killed. Biochemical and molecular biology analyses were carried out.
Results
Ionized calcium‐binding adaptor molecule 1 and glial fibrillary acidic protein expression values in the MS + Pro + Exe, MS + Pro, and MS + Exe groups showed a significant decrease compared with the MS group (P < .05). Also, glial fibrillary acidic protein gene expression in the intervention groups showed an insignificant decrease (P > .05). The IL‐6 and IL‐1β values in the MS + Exe and MS + Pro + Exe groups showed a significant decrease compared with the MS group (P < .05).
Conclusions
Exercise with the intake of probiotics improved demyelination in the cuprizone‐induced demyelination mouse model of MS.
Multiple sclerosis is a common inflammatory disorder of the central nervous system categorized by myelin loss and degeneration of neurons in the brain and spinal cord. Feeding C57/Bl6 mice cuprizone induces reversible demyelination, predominantly of the corpus callosum. In this study, we emphasize exercise training and the consumption of probiotics. Our results showed that in addition to the effect of exercise training and consumption of probiotics on demyelination, both of these interventions have a greater effect on demyelination.