Acid Hydrolysis of Pectin for Cell Growth of Cupriavidus necator
The effect of acid concentration (1-7%, v/v) and temperature (70-100 degree C) on the pectin hydrolysis was studied using pectin at 1% (w/v) for 4 h hydrolysis. Experiments were arranged according to a central composite statistical design and Response Surface Methodology (RSM) was used to assess fac...
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| Published in: | Biotechnology (Faisalābād, Pakistan) Vol. 11; no. 1; pp. 29 - 36 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Online Access: | Get full text |
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| Summary: | The effect of acid concentration (1-7%, v/v) and temperature (70-100 degree C) on the pectin hydrolysis was studied using pectin at 1% (w/v) for 4 h hydrolysis. Experiments were arranged according to a central composite statistical design and Response Surface Methodology (RSM) was used to assess factor interactions and empirical models on response variable (reducing groups concentration - RGC). The highest RGC (6.5 g L super(-1)) was achieved at 100 degree C and 1% (v/v) H sub(2)SO sub(4). Kinetics of RGC release was also performed and hydrolysate was used as carbon source for cell growth of Cupriavidus necator. The maximum specific growth rate and the substrate-to-cell conversion factor were 0.26 h super(-1) and 0.55 g g super(-1), respectively an indicative of that pectin hydrolysate is a potential substrate for cell growth of this microorganism. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1682-296X |
| DOI: | 10.3923/biotech.2012.29.36 |