Antibacterial Ability and Molecular Characterization of Probionts Isolated from Gut Microflora of Cultured Red Tilapia

• Published in: Asian journal of animal and veterinary advances Vol. 8; no. 1; pp. 116 - 123
• Main Authors: Char Yee, Lim, Ina-Salwan, M.Y., M. Abdelha, Yasser
• Format: Journal Article
• Language: English
• Published: 2013
• Subjects: Aeromonas hydrophila; Aeromonas salmonicida; Bacillus megaterium; Paenibacillus; Staphylococcus cohnii; Tilapia; Vibrio alginolyticus
• ISSN: 1683-9919
• DOI: 10.3923/ajava.2013.116.123

Four bacterial isolates, which proved to have the potential to be used as probiotics, were used to study their genotypic characterization using analysis of 16S rRNA gene sequence, antagonistic ability and safety when applied on tilapia via immersion. Gram staining showed that all 4 probionts were Gram positive bacteria. Three probionts were rod in shape and the fourth was cocci in shape and arranged in grape-like cluster. These 4 probionts were also identified using 16S rRNA gene sequencing and their identities were Paenibacillus barcinonensis strain D12, Paenibacillus sp. strain D14, Staphylococcus cohnii strain Bll and Bacillus megaterium strain E28, respectively. All of the probionts were examined for their antagonistic ability against pathogenic bacteria (Vibrio alginolyticus ATCC 33839, Aeromonas salmonicida and Aeromonas hydrophila ATCC 35654) under in vitro conditions by using cross-streaking method. P. barcinonensis strain D12 and Paenibacillus sp. strain D14 have shown stronger antagonistic ability than S. cohnii strain B11 and B. megaterium strain E28 in the antagonism test. P. barcinonensis strain D12 and Paenibacillus sp. strain D14 were chosen to test their safety on tilapia due to their better performance in antagonism test. Both probionts, P. barcinonensisstrain D12 and Paenibacillus sp. strain D14, were safe for tilapia.