Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste
The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the s...
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Published in: | Water science and technology Vol. 38; no. 12; pp. 123 - 126 |
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Main Authors: | , , , , |
Format: | Journal Article |
Language: | English |
Published: |
01-01-1998
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Online Access: | Get full text |
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Summary: | The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment. |
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ISSN: | 0273-1223 1996-9732 |
DOI: | 10.2166/wst.1998.0520 |