Detection and survival of pathogens during two-stage thermophilic/mesophilic anaerobic treatment of suspended organic waste

The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the s...

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Bibliographic Details
Published in:Water science and technology Vol. 38; no. 12; pp. 123 - 126
Main Authors: Burtscher, C., Fall, P. A., Christ, O., Wilderer, P. A., Wuertz, S.
Format: Journal Article
Language:English
Published: 01-01-1998
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Summary:The food-borne pathogens Salmonella typhimurium and Listeria monocytogenes were effectively eliminated from suspended organic waste during the thermophilic (55°C) phase of a two-stage thermophilic/mesophilic anaerobic reactor. The feasibility of PCR technology was evaluated in order to improve the speed of detection and reduce the effort involved in detecting the presence of a range of pathogenic bacteria. Our results show direct extraction of nucleic acids followed by purification and PCR to be a promising method that will enable the rapid verification of hygienic performance during waste treatment.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.1998.0520