Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

• Published in: Pakistan journal of biological sciences Vol. 18; no. 6; pp. 279 - 284
• Main Authors: Elya, Berna, Handayani, Rosita, Sauriasari, Rani, ., Azizahwati, Hasyyati, Uqie Shabrina, Permana, Idam Titis, Permatasar, Yunita Indah
• Format: Journal Article
• Language: English
• Published: 2015
• Subjects: Artocarpus; Lagerstroemia speciosa; Persea americana; Syzygium
• ISSN: 1028-8880; 1812-5735
• DOI: 10.3923/pjbs.2015.279.284

Active compounds of plants have potency as antidiabetic that can be used in diabetes mellitus treatment. The aim of this study was to determine in vitro inhibitory activity of alpha -glucosidase, alpha -amylase and Dipeptidyl peptidase IV from 42 ethanolic extracts of selected Indonesian plants. Inhibitory activity was measured using spectrophotometric method. The alpha -amylase activity was measured at lambda = 540 nm, alpha -glucosidase activity and the DPP-IV activity were measured at lambda = 405 nm. Eight extracts inhibit both alpha -glucosidase and alpha -amylase better than acarbose as a positive control. These extracts are Camelia sinensis (white, green, fermented), Pometia pinnata, Syzygium polyanthum, Artocarpus heteropyllus, Lagerstroemia speciosa and Persea Americana. The ethanolic extracts have DPP-IV inhibitory activity between 10-20%, lower than Diprotin as positive control, except Camelia sinensis (white) which gave the inhibition more than 30% at 62.5 mu g mL super( -1). This study showed us that some extracts have inhibition activity of alpha -glucosidase, alpha -amylase and DPP-IV.