Colorimetric detection of neomycin sulfate in serum based on ultra-small gold nanoparticles with peroxidase-like activity

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 321; p. 124686
• Main Authors: Zang, Wen, Peng, Minjie, Yang, Yiyu, Zhang, Chenguang, Liu, Zhusheng, Wang, Le, Wang, Chaozhen, Lin, Jie, Chen, Tianxiang, Zhang, Yujie, Li, Juan, Wu, Aiguo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15-11-2024
• Subjects: Colorimetric detection; Gold nanoparticles; Neomycin sulfate; Peroxidase-like activity; Serum; Serum; Peroxidase-like activity; Neomycin sulfate; Gold nanoparticles; Colorimetric detection
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124686

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•NEO detection was achieved by utilizing enhanced peroxidase activity mechanism caused by the aggregation of PVP/Au NPs.•The colorimetric probe can be applied to the detection of NEO in serum and has a broad application prospect.•The detection limit of NEO probe is 50 nM by naked eyes and 1 nM by UV-visible absorption spectra.•PVP/Au NPs based colorimetric sensor for NEO is simple, sensitive and selective.
Neomycin sulfate (NEO) is a kind of aminoglycoside antibiotics. Because of its strong ototoxicity, nephrotoxicity and other side effects, its content in the body should be strictly monitored during use. In this paper, a rapid colorimetric detection method for NEO based on ultrasmall polyvinylpyrrolidone modified gold nanoparticles (PVP/Au NPs) with peroxidase-like activity was developed. Firstly, ultra small PVP/Au NPs with weak peroxidase-like activity were synthetized. When they were mixed with NEO, strong hydrogen bonds were formed between NEO and PVP, resulting in the aggregation of PVP/Au NPs, and the aggregated PVP/Au NPs showed stronger peroxidase-like activity. Therefore, rapid colorimetric detection of NEO was achieved by utilizing the enhanced peroxidase-like activity mechanism caused by the aggregation of ultra small PVP/Au NPs. The naked eye detection limit of this method is 50 nM. Within the range of 1 nM-300 nM, there was a good linear relationship between NEO concentration and the change in absorbance intensity of PVP/Au NPs-H2O2-TMB solution at 652 nm, with the regression curve of y = 0.0045x + 0.0525 (R2 = 0.998), and the detection limit is 1 nM. In addition, this method was successfully applied to the detection of NEO in mouse serum. The recoveries were 104.4 % −107.6 % compared with HPLC assay results, indicating that this method for NEO detection based on PVP/Au NPs has great potential in actual detection of NEO in serum.