Evaluation of effects of bisphenol analogs AF, S, and F on viability, proliferation, production of selected cancer-related factors, and expression of selected transcripts in Caov-3 human ovarian epithelial cell line

• Published in: Food and chemical toxicology Vol. 191; p. 114889
• Main Authors: Bujnakova Mlynarcikova, Alzbeta, Scsukova, Sona
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-09-2024
• Subjects: Bisphenol analogs; Interleukins; Ovarian cancer; Reactive oxygen species; Vascular endothelial growth factor; Bisphenol analogs; Reactive oxygen species; Interleukins; Vascular endothelial growth factor; Ovarian cancer
• ISSN: 0278-6915; 1873-6351; 1873-6351
• DOI: 10.1016/j.fct.2024.114889

Bisphenol A (BPA) has been a substantial additive in plastics until the reports on its adverse effects have led to its restrictions and replacement. Monitoring studies document the increasing occurrence of bisphenol analogs, however, data on their effects and risks is still insufficient. Based on the indications that BPA might contribute to ovarian cancer pathogenesis, we examined effects of the analogs AF (BPAF), S (BPS) and F (BPF) (10−9–10−4 M) on the Caov-3 epithelial cancer cells, including the impact on cell viability, proliferation, oxidative stress, and production and expression of several factors and genes related to ovarian cancer. At environmentally relevant doses, bisphenols did not exert significant effects. At the highest concentration, BPAF caused varied alterations, including decreased cell viability and proliferation, caspase activation, down-regulation of PCNA and BIRC5, elevation of IL8, VEGFA, MYC, PTGS2 and ABCB1 expressions. Only BPA (10−4 M) increased IL-6, IL-8 and VEGFA output by the Caov-3 cells. Each bisphenol induced generation of reactive oxygen species and decreased superoxide dismutase activity at the highest concentration. Although the effects were observed only in the supraphysiological doses, the results indicate that certain bisphenol analogs might affect several ovarian cancer cell characteristics and merit further investigation.
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•Bisphenols exerted alterations of some ovarian cancer cell features•BPAF disrupted Caov-3 cell viability via increased ROS and caspase activities•BPAF dysregulated expression of several genes (PCNA, BIRC5, VEGFA, MYC, ABCB1)•BPA stimulated IL-6, IL-8, and VEGF-A production without affecting cell viability•Bisphenol impacts were observed only at supraphysiological concentration (10−4 M)