Transcriptional analysis of WUSCHEL-related HOMEOBOX (WOX) genes in Coffea arabica L

Transcriptional analysis of WUSCHEL-related HOMEOBOX (WOX) genes in Coffea arabica L

Growing coffee faces challenges regarding the development and productivity of plants. To improve coffee tree cultivation and bean productivity and quality, modern techniques of plant biotechnology need to be adopted that allow the development of more productive and resistant varieties. Among these,...

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Bibliographic Details
Published in:Biológia Vol. 75; no. 9; pp. 1483 - 1495
Main Authors: Daude, Matheus Martins, Dos Santos Silva, Thyeiry Winny, Freitas, Natália Chagas, Ságio, Solange Aparecida, Paiva, Luciano Vilela, Barreto, Horllys Gomes
Format: Journal Article
Language:English
Published: Cham Springer International Publishing 01-09-2020
Springer Nature B.V
Subjects:
Biomedical and Life Sciences
Biotechnology
Cell Biology
Coffea arabica
Coffee
Cultivation
Embryonic growth stage
Gene expression
Genes
Homeobox
Life Sciences
Microbiology
Original Article
Pattern analysis
Plant Sciences
Productivity
Propagation
Somatic embryogenesis
Transcription
Zoology
Real time PCR
Molecular marker
Gene expression
Bioinformatics
Somatic embryogenesis
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Summary:Growing coffee faces challenges regarding the development and productivity of plants. To improve coffee tree cultivation and bean productivity and quality, modern techniques of plant biotechnology need to be adopted that allow the development of more productive and resistant varieties. Among these, somatic embryogenesis stands out as a method of great potential for the propagation of coffee plants in vitro. Some genes are directly related to somatic embryogenesis, especially WOX (WUSHEL-related HOMEOBOX) genes, which play a central role in the process. Therefore, the objective of this study was to identify and analyze the expression pattern of WOX genes in arabica coffee, Coffea arabica . In silico analyses identified seven WOX genes in arabica coffee: Coffea arabica WUSHEL-like (CaWUS-like) , Coffea arabica WOX1-like (CaWOX1-like) , CaWOX3-like , CaWOX4-like , CaWOX5-like , CaWOX9-like and CaWOX11-like . The transcriptional profile of the CaWOX11-like gene suggests that it may be intrinsically related to the embryogenic process and may be used as a molecular marker. In addition, comparisons of the present results with those from the literature indicate that WOX genes are key regulators of somatic embryogenesis in arabica coffee.
ISSN:0006-3088
1336-9563
DOI:10.2478/s11756-020-00460-8