Expression and its clinical significance of HLA-G in HCMV-infected placental villi at early pregnant stage

• Published in: Journal of Medical Colleges of PLA Vol. 22; no. 1; pp. 31 - 35
• Main Author: 张欣文 李芬 盛秋 于学文 任永惠 李学成
• Format: Journal Article
• Language: English
• Published: Maternal and Child Health Center,First Affiliated Hospital of Medical College,Xi'an Jiaotong University,Xi'an 710061,China 01-02-2007
• Subjects: HLA-G; 基因表达; 妊娠早期; 子宫内感染; 巨细胞病毒感染; 胎盘绒毛; intrauterine infection; maternal-fetal interface; human cytomegalovirus; HLA-G
• ISSN: 1000-1948
• DOI: 10.1016/S1000-1948(07)60007-0

Objective：To study the expression and its clinical significance of HLA-G in HCMV intrauterine infected placental villi at early pregnant stage. Methods：PCR （polymerase chain reaction） was used to screen the peripheral blood for HCMV-DNA in 462 women who had willingly undergone induced abortion. Then immunohistochemistry was also used to detect expressions of mouse anti-HCMV early antigen （HCMV-EA） and mouse anti-HLA-G in HCMV-DNA positive cases＇ placental villi. The difference of HLA-G expressions between the intrauterine infection group（HCMV-EA positives）, the intrauterine infection-free group（HCMV-EA negatives） and the normal control group （50 cases of healthy early placental villi） was compared. Results： Of the 78 cases, which were detected HCMV-DNA positive, 11 （14.10%） were HCMV-EA positive. Compared with the other two groups, HLA-G expressions in the intrauterine infection group were both obviously decreased（both P〈0. 001）. HLA-G expression positions in all three groups were mainly located in the cytotrophoblast. Conclusion：Intrauterine HCMV infection at early pregnant stage is closely related to HLA-G expression at the maternal-fetal interface. The virogenetic products may affect the expression of HLA-G at the maternal-fetal interface and that of its immunological function, thus leading to different clinical outcomes.