Intraspecific Variation in Gloriosa Superba (L.) Population from Western Himalayas (India) and Evaluation of in vitro Antigout Potential

• Published in: National Academy of Sciences, India. Proceedings. Section B. Biological Sciences Vol. 92; no. 2; pp. 473 - 483
• Main Authors: Misra, Ankita, Shukla, Pushpendra Kumar, Kumar, Manish, Rawat, Poonam, Srivastava, Sharad
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New Delhi Springer India 01-06-2022; Springer Nature B.V
• Subjects: Behavioral Sciences; Biomedical and Life Sciences; Colchicine; Germplasm; Life Sciences; Nucleic Acid Chemistry; Plant Biochemistry; Research Article; Variation; Himalaya Mountains; India; Chemotaxonomy; Gouty arthritis; Colchicine
• ISSN: 0369-8211; 2250-1746
• DOI: 10.1007/s40011-022-01350-6

The natural population(s) of
Gloriosa superba
L. was mapped from 19 different locations of Western Himalayas, up to altitude of 1800 m and no significant morphotypic variation was observed. The major metabolite colchicine was quantified through HPTLC (validated) method and significant (
p
 < 0.05) intraspecific variation was recorded. The colchicine content ranges from 0.023 to 0.256% and maximum was in NBG-03 from Jyolikot, Nainital (Uttarakhand, India). Total polyphenolics content was also significant among the collected samples. Cluster analysis based on colchicine and total polyphenolic content of the population’s results in identification of five elite chemotypes viz. NBG-03, NBG-54, NBG-57, NBG-65 and NBG-80. The
in vitro
antigout activity of elite germplasms reveals that NBG-57 was the most promising, although its colchicine content was in moderation. Therefore, it is suggested that the whole extract may exert more promising antigout potential due to the synergistic action of various phytomolecules. Hence, the study will promote the conservation and cultivation of targeted species in Western Himalayas for the availability of good quality raw material for industry. It will also aid in commercial cultivation of elite germplasm(s) which can be used in herbal product development as well as in the economic upliftment of local inhabitants.