Simultaneous detection of fumonisin B1 and ochratoxin A using dual-color, time-resolved luminescent nanoparticles (NaYF4: Ce, Tb and NH2-Eu/DPA@SiO2) as labels

• Published in: Analytical and bioanalytical chemistry Vol. 411; no. 7; pp. 1453 - 1465
• Main Authors: Niazi, Sobia, Khan, Imran Mahmood, Yan, Lv, Khan, Muhammad Issa, Mohsin, Ali, Duan, Nuo, Wu, Shijia, Wang, Zhouping
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2019; Springer Nature B.V
• Subjects: Analytical Chemistry; Aptamers; Biochemistry; Cerium; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Color; Corn; Deoxyribonucleic acid; DNA; Enzyme-linked immunosorbent assay; Fluorescence; Fluorides; Food safety; Food Science; Fumonisin B1; Iron oxides; Laboratory Medicine; Monitoring/Environmental Analysis; Mycotoxins; Nanoparticles; Ochratoxin A; Recognition; Research Paper; Silicon dioxide; Sodium compounds; Terbium; Toxins; Aptamer; Simultaneous detection; Time-resolved fluorescence nanoparticles; Mycotoxins
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-019-01580-0

A rapid and highly sensitive time-resolved fluorescence (TRF)-based aptasensor for simultaneous recognition of mycotoxins ochratoxin A (OTA) and fumonisin B
1
(FB
1
) was developed using multi-color, Ln
3+
-doped time-resolved fluorescence nanoparticles (TRF-NPs) (NaYF
4
: Ce, Tb and NH
2
-Eu/DPA@SiO
2
NPs) coupled with complementary strand DNA (cDNA) as luminescence probe and aptamers-conjugated amine-functionalized Fe
3
O
4
magnetic nanoparticles (MNPs) act as a capture probe. Under the optimized conditions, the time-resolved fluorescence intensities at 544 and 618 nm corresponded with Tb
3+
and Eu
3+
, respectively, were used to measure FB
1
(
Y
 = 19,177.1 + (− 12,054.4)
x
,
R
2
 = 0.9917) and OTA (
Y
 = 4138.8 + (− 11,182.6)
x
,
R
2
 = 0.9924), respectively. The limits of detection (LODs) for FB
1
and OTA were 0.019 pg mL
−1
and 0.015 pg mL
−1
, respectively, which were much lower than previously described methods for simultaneous recognition of mycotoxins OTA and FB
1
while detection range varied from 0.0001–0.5 ng mL
−1
. This aptasensor was effectively applied to quantity FB
1
and OTA in maize samples and results were compared with ELISA method. This is the first reported time-resolved fluorescence (TRF)-based aptasensor to detect two agriculturally important toxins in the maize. The developed aptasensor has potential to be used for detection of toxins in food safety fields.
Graphical abstract