Cytokine-regulating activity of anti-virus preparation CelAgripus in Burkitt's lym-phoma stable B-cell lines

• Published in: Voprosy virusologiĭ Vol. 64; no. 4; pp. 165 - 172
• Main Authors: Narovlyansky, A. N., Mezentseva, M. V., Suetina, I. A., Russu, L. I., Ivanova, A. M., Poloskov, V. V., Izmest'eva, A. V., Ospelnikova, T. P., Sarymsakov, A. A., Ershov, F. I.
• Format: Journal Article
• Language: English
• Published: Central Research Institute for Epidemiology 20-08-2019
• Subjects: burkitt’s lymphoma; cytokines; epstein-barr virus; gene expression; interferon inducer; stable cell lines; the polymerase chain reaction
• ISSN: 0507-4088; 2411-2097
• DOI: 10.36233/0507-4088-2019-64-4-165-172

Introduction. Cytokines activated in response to immunosuppressive viral infections can directly or indirectly affect the neoplastic transformation of B cells. In this study, we studied a new substance designed to produce the antiviral drug CelAgrip (CA, CelAgripus), which exhibits interferon (IFN) and cytokine-inducing activity and, apparently, can be used as an activator of antiviral immunity.Purpose - is to evaluate the cytokine-regulating effect of CA in Burkitt's lymphoma (LB) cell lines latently infected with the Epstein-Barr virus (EBV).Objectives: to study the CA-induced expression of the cytokine genes IL-ip, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-18, IFN-a, IFN -Y, IFN-p, IFN-A1, IFN-A2, IFN-A3, TNF-a in normal and EBV transformed LB cells.Material and methods. Cell line: the human embryo fibroblasts (HEF), Namalva, Daudi, Raji, P3HR-1. Preparations: CA, gossypol-acetic acid (GAA), sodium carboxymethyl cellulose (Na-CMC). Methods: RT-PCR and methods for assessing cytotoxicity (MTT and Scepter 2.0 Merck cell counter).Results. The effect of the CA preparation on the expression of IFN-Л, IL-1p, IL-6, IL-8 and IL-10 genes was revealed. Discussion. We observed the activation of gene expression of IFN-A, IL-1P, IL-6, IL-8 and suppression of IL-10 gene activity when treatment CA of LB cells.Conclusion. The substance CA has new effects on the activation of the expression of a number of key cytokine genes in stable Burkitt lymphoma cell lines.