Improvement of Myocardial Structure after Developed Fibrous Degeneration Under the Use of Allogenic Biomaterial

• Published in: Razrabotka i registraciâ lekarstvennyh sredstv (Online) Vol. 12; no. 3; pp. 202 - 211
• Main Authors: Lebedeva, A. I., Afanasiev, S. A., Gareev, E. M., Kondratieva, D. S., Musina, L. A., Popov, S. V., Prusakov, A. V., Yashin, A. V., Ponamarev, V. S., Radnatarov, V. D.
• Format: Journal Article
• Language: English
• Published: LLC Center of Pharmaceutical Analytics (LLC «CPHA») 01-09-2023
• Subjects: allogeneic biomaterial; cardiomyogenic cells; extracellular extracellular matrix; myocardium; regeneration; scar
• ISSN: 2305-2066; 2658-5049
• DOI: 10.33380/2305-2066-2023-12-3-202-211

Introduction.
The question of the possibility of recovery of postischemic myocardium remains relevant.
Aim.
The aim of the study was to study the effect of dispersed decellularized allogeneic extracellular matrix (allogeneic biomaterial, DAB) on the developed fibrous degeneration of the myocardium, as well as to reveal the possible mechanisms of cellular regeneration.
Materials and methods.
The muscular wall of the heart of rats was subjected to cryodestruction. After 45 days, the rats of the main group were intramyocardially injected with a suspension of allogeneic biomaterialinto the area of the affected myocardium, and the rats of the control group were injected with saline.
Results and discussions.
In the experimental group, there was a regression of the formed fibrous connective tissue, chemoattraction of progenitor cells, their differentiation and integration into the myocardium. The thickness of the muscular part of the wall of the left ventricle was three orders of magnitude higher than in the control group.
Conclusion.
Analysis of the results of the study indicates that the heart in adult mammals has a powerful regenerative reserve. It is likely that, based on the use of DAB, a protocol can be developed that allows the restoration of the heart muscle even in conditions of already developed fibrous degeneration.