Toxicity of miR-204-5p Inhibition for Melanoma B16 Cells in vitro and Mice in vivo

• Published in: Cell and tissue biology Vol. 12; no. 4; pp. 307 - 314
• Main Authors: Palkina, N. V., Komina, A. V., Aksenenko, M. B., Belonogov, R. N., Lavrentev, S. N., Ruksha, T. G.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-07-2018; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Cancer; Cell Biology; Cell proliferation; Kinases; Life Sciences; Lysine; Melanoma; MicroRNAs; miRNA; Pluripotency; Signal transduction; Stem cell transplantation; Stem cells; Toxicity; Transcription; Transfection; melanoma B16; signaling pathways; MTT; LNA™ inhibitors; miR-204-5p
• ISSN: 1990-519X; 1990-5203
• DOI: 10.1134/S1990519X18040077

MiR-204-5p is an oncosuppressive microRNA the level of which diminishes in various cancer types. The aim of this study was to evaluate miR-204-5p inhibition of melanoma-cell viability, as well as to determine whether miR-204-5p transfection of melanoma B16 cells with miR-204-5p mimic/inhibitor affected the microRNA expression. Proliferation of transfected cells was slightly, probably nonspecifically, reduced after 96 h. LNA™ inhibitors produced toxic effects in vivo. miR-204-5p-mimic/inhibitor application resulted to a slight, most likely nonspecific, modulation of melanoma-cell proliferation after 96 h. Bioinformatic analysis revealed that miR-204-5p is involved in the estrogen signaling pathway, lysine degradation, signaling pathways regulating pluripotency of stem cells, melanogenesis, transcriptional deregulation in cancer. Injection of miR-204-5p LNA™ inhibitor into mice reduced the level pf miR-204-5p but was not toxic.