Water-soluble C60 fullerenes reduce manifestations of acute cholangitis in rats

• Published in: Applied nanoscience Vol. 9; no. 5; pp. 601 - 608
• Main Authors: Kuznietsova, H. M., Lynchak, O. V., Dziubenko, N. V., Osetskyi, V. L., Ogloblya, O. V., Prylutskyy, Yu I., Rybalchenko, V. K., Ritter, U., Scharff, P.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-07-2019; Springer Nature B.V
• Subjects: Alanine; Alkaline phosphatase; Animals; Atrophy; Biocompatibility; Buckminsterfullerene; Chemistry and Materials Science; Computer simulation; Creatinine; Epithelium; Etiology; Fibrosis; Fullerenes; Gallbladder diseases; Ingestion; Lactate dehydrogenase; Liver; Materials Science; Membrane Biology; Nanochemistry; Nanotechnology; Nanotechnology and Microengineering; Original Article; Rats; Rodents; Water chemistry; C; Liver enzymes activity; STM and AFM measurements; Liver morphology; Acute cholangitis; fullerene
• ISSN: 2190-5509; 2190-5517
• DOI: 10.1007/s13204-018-0700-5

Sclerosing cholangitis is the liver disease of uncertain etiology, extremely unfavorable prognosis and lack of effective medication therapy. Therefore, the effect of water-soluble biocompatible C
60
fullerenes (C
60
FAS) on the liver functional state on rat acute-cholangitis model was aimed to be discovered. Acute cholangitis was simulated by single α-naphthyl isothiocyanate (ANIT, 100 mg/kg) per os administration; C
60
FAS (0.5 mg/kg) was administered either per os or intraperitoneally in 24 and 48 h after ANIT ingestion, and in 72 h the animals were sacrificed. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), the total and direct bilirubin, creatinine and urea in the blood serum were determined, and the liver morphological state was assessed. In animals experienced ANIT-induced acute cholangitis, the total and direct bilirubin, creatinine, ALT, AST, ALP and LDH 1.5–4-fold increase were observed, indicating cytolysis of hepatocytes, cholestasis, and renal dysfunction. The features of periductal fibrosis, biliary epithelium atrophy, and portal–portal linking septa formation were detected, confirming the sclerosing cholangitis development. C
60
FAS promoted to the normalization of direct and total bilirubin levels, the ALT activity and diminution of fibrotic features. In addition, C
60
FAS intraperitoneal administration also normalized the ALP activity, indicating the attenuation of disease symptoms. However, the AST activity and creatinine level remained unchanged, and the LDH activity even increased, manifesting the partial persistence of cholestasis and renal dysfunction. Thus, the therapeutic application of C
60
FAS promotes a partial protection of liver against cholangitis.