Platelet adhesion to collagen and endothelial cell matrix under flow conditions is not dependent on platelet glycoprotein IV

• Published in: Blood Vol. 83; no. 11; pp. 3240 - 3244
• Main Authors: SAELMAN, E. U. M, KEHREL, B, HESE, K. M, DE GROOT, P. G, SIXMA, J. J, NIEUWENHUIS, H. K
• Format: Journal Article
• Language: English
• Published: Washington, DC The Americain Society of Hematology 01-06-1994
• Subjects: Biological and medical sciences; Blood coagulation. Blood cells; Collagen - physiology; Endothelium, Vascular - cytology; Fundamental and applied biological sciences. Psychology; Humans; Molecular and cellular biology; Platelet; Platelet Adhesiveness; Platelet Membrane Glycoproteins - physiology; Endothelial cell; Platelet; Hemostasis; Collagen; Extracellular matrix; Glycoproteins; Adhesion; Cell surface
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V83.11.3240.3240

Platelet membrane glycoprotein IV (GPIV) is a cell-surface glycoprotein that has been proposed as a receptor for collagen. Recently, it has been shown that platelets with the Naka-negative phenotype lack GPIV on their surface, whereas donors with this phenotype are healthy and do not suffer from hematologic disorders. In this study, we compared Naka-negative platelets with normal platelets in adhesion to collagen types I, III, IV, and V and the extracellular matrix of endothelial cells (ECM) under static and flow conditions. No differences in platelet adhesion and subsequent aggregate formation on the collagens types I, III, and IV were observed under static and flow conditions. Adhesion of both homozygous and heterozygous Naka-negative platelets to collagen type V was strongly reduced under static conditions. Collagen type V was not adhesive under flow conditions. No difference in platelet adhesion to ECM was observed, which suggests that GPIV is not important in adhesion to subendothelium, for which ECM may serve as a model. These results indicate that GPIV is not a functional receptor for collagen under flow conditions.