A non-invasive method for the routine-estimation of fresh weight of cells grown in batch suspension cultures

• Published in: Plant cell reports Vol. 11; no. 3; pp. 146 - 149
• Main Authors: BLOM, T. J. M, KREIS, W, VAN IREN, F, LIBBENGA, K. R
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01-04-1992
• Subjects: Biological and medical sciences; Biotechnology; Establishment of new cell lines, improvement of cultural methods, mass culture; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Plant cells and fungal cells; Cell culture; Suspension culture; Vegetals; Batchwise; Biomass; Cell volume; Sedimentation; Quantitative analysis
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/BF00232168

A simple apparatus was designed to allow sedimentation of plant cells grown in batch suspensions in Erlenmeyer flasks. After sedimentation the height of the cell mass along the glass wall was measured with a ruler fixed in the apparatus. The cell volume after sedimentation, calculated from this height, appeared highly correlated with the fresh weight of cells. This result was found with eight cell lines in two Laboratories. The method proved to be very suitable to allow routinely measurement of FW without the destruction of cells, from many samples, in a short time, during each phase of the growth cycle.