Effects of linoleic and gamma-linolenic acids (efamol evening primrose oil) on fatty acid-binding proteins of rat liver

• Published in: Molecular and cellular biochemistry Vol. 98; no. 1-2; pp. 177 - 182
• Main Authors: Dutta-Roy, A K, Demarco, A C, Raha, S K, Shay, J, Garvey, M, Horrobin, D F
• Format: Journal Article
• Language: English
• Published: Netherlands 01-10-1990
• Subjects: Animals; Carrier Proteins - metabolism; Cholesterol Esters - metabolism; Eating; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; Fatty Acids, Essential - pharmacology; Female; gamma -linoleic acid; gamma-Linolenic Acid; Humans; Hypolipidemic Agents - pharmacology; Linoleic Acid; Linoleic Acids - pharmacology; Linolenic Acids - pharmacology; Liver - drug effects; Liver - metabolism; Neoplasm Proteins; Nerve Tissue Proteins; Phospholipids - metabolism; Plant Oils; Rats; Rats, Inbred Strains; Triglycerides - metabolism; Tumor Suppressor Proteins
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/BF00231382

We have studied the effects of Efamol evening primrose oil (EPO) on fatty acid-binding proteins (L-FABP) of rat liver. EPO contains 72% cis-linoleic acid and 9% cis-gamma linolenic acid. EPO has been clinically used for treatment of a number of diseases in humans and animals. EPO is also known to lower cholesterol level in humans and animals. Feeding of an EPO supplemented diet to rats (n = 9) for 2 months decreases the oleate binding capacity of purified L-FABP of rat liver whereas the palmitate binding activity was increased by 38%. However, EPO feeding did not alter the L-FABP concentrations significantly as measured by using the fluorescence fatty acid probe, dansylamino undecanoic acid. Endogenous fatty acid analysis of L-FABPs revealed significant qualitative and quantitative changes in fatty acid pattern after EPO feeding. EPO feeding decreased the endogenous palmitate level by 53% and oleate level by 64% in L-FABPs and also EPO feeding decreased the total endogenous fatty acid content from 62 nanomole per mg of protein to 42 nanomole per mg of L-FABP (n = 3).