Characterization of alpha-adrenergic receptors in guinea pig cerebral cortex: effect of chronic antidepressant treatments

Alpha-adrenergic receptor sites in cerebral cortex membranes of the guinea pig have been characterized by the specific binding of 3H-WB4101, an alpha-adrenergic antagonist. The binding was rapid, reversible, saturable, and stereoselective. The maximal binding site (Bmax) and dissociation constant (k...

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Bibliographic Details
Published in:	Psychopharmacology Vol. 74; no. 3; pp. 201 - 203
Main Authors:	Hu, H Y, Davis, J M, Pandey, G N
Format:	Journal Article
Language:	English
Published:	Germany 1981
Subjects:	Adrenergic alpha-Agonists - pharmacology Adrenergic alpha-Antagonists - pharmacology Animals Antidepressive Agents - pharmacology Binding, Competitive Cerebral Cortex - drug effects Dioxanes Guinea Pigs Male Receptors, Adrenergic - drug effects Receptors, Adrenergic, alpha - drug effects
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Summary:	Alpha-adrenergic receptor sites in cerebral cortex membranes of the guinea pig have been characterized by the specific binding of 3H-WB4101, an alpha-adrenergic antagonist. The binding was rapid, reversible, saturable, and stereoselective. The maximal binding site (Bmax) and dissociation constant (kd) for 3H-WB4101 were calculated to be 251 fmol/mg protein and 1.23 nM, respectively. Ligand displacement experiments suggest that 3H-WB4101 binds to alpha 1-adrenergic receptors. Chronic treatment of guinea pigs with desipramine, amitriptyline, phenelzine, or electroconvulsive shock failed to show any statistically significant change in alpha-adrenergic receptor sensitivity as measured by the binding of 3H-WB4101.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0033-3158 1432-2072
DOI:	10.1007/BF00427093