Fibrinolytic and collagenolytic activity of extracellular proteinases of the strains of micromycetes Aspergillus ochraceus L-1 and Aspergillus ustus 1

• Published in: Moscow University biological sciences bulletin Vol. 71; no. 1; pp. 62 - 66
• Main Authors: Osmolovskiy, A. A, E. A. Popova, V. G. Kreyer, N. A. Baranova, N. S. Egorov
• Format: Journal Article
• Language: English
• Published: New York Allerton Press 2016
• Subjects: Aspergillus ochraceus; Biochemistry; Biomedical and Life Sciences; Cell Biology; collagen; culture media; enzymatic reactions; fibrin; Life Sciences; Microbiology; Plant Sciences; proteinases; proteolysis; sodium nitrate; Zoology; collagenolytic enzymes; fibrinolytic enzymes; proteinases of micromycetes
• ISSN: 0096-3925; 1934-791X
• DOI: 10.3103/S0096392516010053

It was shown that extracellular proteinases produced by the strains of micromycetes A. ochraceus L-1 and A. ustus 1 differ by the activity at various pH as well as by the intensity of the effect on fibrillar proteins. It was revealed that the proteinases of A. ochraceus L-1 demonstrated maximum activity during the growth of the producer in the nitrate-free growth medium (the pH of enzyme reaction was 8.0), whereas those of A. ustus 1 showed maximal activity during the growth of the micromycete in the medium containing sodium nitrate (the pH of enzyme reaction was 6.0). Values of specific fibrinolytic and collagenolytic activities of A. ochraceus L-1 were 2.2 and 1.6 times higher than those of A. ustus 1. At the same time, A. ustus 1 showed very low values of total proteolytic (caseinolytic) activity and had a high ratio of fibrinolytic activity to total proteolytic (caseinolytic) activity (6.92). It makes the strain a promising producer of proteinases, which hydrolyze fibrin and collagen.