Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum

Continuous culture growth of photoautotrophic cell suspensions from Chenopodium rubrum

The construction and operation of a continuous culture system for the propagation of cell suspensions from Chenopodium rubrum under photoautotrophic conditions has been described. A dilution rate of 0.16/day gave an equilibrium culture density of 1,100,000 cells/ml and a mean doubling time of 150 ho...

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Bibliographic Details
Published in:Plant cell reports Vol. 2; no. 2; pp. 59 - 62
Main Author: Husemann, W
Format: Journal Article
Language:English
Published: Germany 01-04-1983
Subjects:
plant biochemistry
plant physiology
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Summary:The construction and operation of a continuous culture system for the propagation of cell suspensions from Chenopodium rubrum under photoautotrophic conditions has been described. A dilution rate of 0.16/day gave an equilibrium culture density of 1,100,000 cells/ml and a mean doubling time of 150 hours. During continuous culture steady state conditions with respect to nutrient uptake, cell protein and chlorophyll content, starch accumulation, in vitro activities of enzymes related to different metabolic pathways could be established. Photosynthetic CO2 assimilation of steady state cells was about 100 mol CO2/mg chlorophyll x hour. Dark CO2 fixation was 3% of the light values.
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content type line 23
ISSN:0721-7714
1432-203X
DOI:10.1007/BF00270165