MTHFR C677T and A1298C Gene Polymorphisms in Human Kidney Cancer Tissues

• Published in: İstanbul Medical Journal Vol. 20; no. 5; pp. 408 - 412
• Main Authors: Pençe, Halime Hanım, Çaykara, Burcu, ALsaadoni, Hani, Ötünçtemur, Alper, Pençe, Sadrettin
• Format: Journal Article
• Language: English
• Published: Galenos Yayinevi 01-09-2019
• Subjects: a1298c; c677t; kidney cancer; mthfr; polymorphism
• ISSN: 2619-9793; 2148-094X
• DOI: 10.4274/imj.galenos.2019.88709

Introduction:The potential effect of 5,10-methylenetetrahydrofolate reductase (MTHFR) on DNA methylation, DNA repair and DNA synthesis has made MTHFR a cancer-inducing gene. In this study, we aimed to evaluate C677T and A1298C gene polymorphisms in kidney cancer.Methods:During the normal treatment procedure, 100 tumor and 100 surrounding healthy kidney tissue samples were obtained from patients after surgery. DNA was isolated from the tissues by DNA Isolation kit. Polymerase chain reaction and restriction fragment length polymorphism methods were used. Genotype and allele distributions were analyzed using SPSS 22 and p<0.05 was considered statistically significant.Results:No significant difference was found between the genotypes and alleles of MTHFR C677T and A1298C polymorphisms in tumor and control groups (p>0.05). MTHFR C677T CC genotype (51%) was found to be higher in kidney cancer tissues than CT genotype (34%). Odds ratio of MTHFR C677T CC genotype was found 1.8 compared to CT genotype (p<0.05).Conclusion:Our findings indicate that MTHFR C677T polymorphism may be effective in normal genotype in kidney cancer tissues.