Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents

Staining Procedure of Cartilage and Skeleton in Adult Bats and Rodents

Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This techni...

Full description

Saved in:
Bibliographic Details
Published in:International journal of morphology Vol. 27; no. 4; pp. 1163 - 1167
Main Authors: Cortés-Delgado, Natalia, Pérez-Torres, Jairo, Hoyos, Julio Mario
Format: Journal Article
Language:English
Published: Sociedad Chilena de Anatomía 01-12-2009
Subjects:
ANATOMY & MORPHOLOGY
Cartilage
Morphology
Rodentia
Hueso
Bone
Cartílago
Morfología
Chiroptera
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transparency technique to demonstrate cartilage and skeleton has been used from years 70. This consists basically of the muscle digestion through an enzyme (trypsine), and the staining of cartilage and skeleton using alcian blue and alizarin red on specimens previously fixed to formalin. This technique can be used to study the state of the cartilage and the bones in different stages of the development and to observe abnormalitys. Also it can be used to demonstrate museum specimen skeleton preserved in liquid. In this article is showed the application of this technique for the first time in rodents and adult bats, besides some annotations to optimize the procedure.
ISSN:0717-9502
0717-9502
DOI:10.4067/S0717-95022009000400032