On the issue of obtaining platelet-rich plasma

• Published in: Innovacionnaâ medicina Kubani (Online) no. 1; pp. 38 - 43
• Main Authors: Bazlov, S. B., Melkonian, K. I., Rusinova, T. V., Popandopulo, K. I., Marchenko, N. V., Shevchuk, D. D.
• Format: Journal Article
• Language: English
• Published: Scientific Research Institute, Ochapovsky Regional Clinical Hospital no. 1 29-03-2022
• Subjects: clinical efficacy; growth factors; obtaining method; platelet-rich plasma; prp; standardisation
• ISSN: 2500-0268; 2541-9897
• DOI: 10.35401/2500-0268-2022-25-1-38-43

Objective:
To determine the optimal technological modes for the preparation of platelet-rich plasma (PRP) using standard laboratory equipment.
Material and methods:
Blood for the research was taken from 25 healthy volunteers. Its centrifugation was performed on a standard CM-6M laboratory centrifuge using various modes and two types of vacuum tubes with lithium heparin containing separation gel and without it. The number of platelets and leukocytes was calculated in the upper, lower and middle layers of the obtained plasma sample.
Results:
Plasma samples obtained during centrifugation modes from 415 to 1660 g for 10 minutes using test tubes that do not contain separation gel are optimal in terms of the number of platelets. Plasma intake from the lower layer of the obtained sample after centrifugation is always accompanied by the inclusion of leukocytes in its composition, which can lead to undesirable tissue reactions when it is used.
Conclusion:
To obtain PRP, it is possible to use standard laboratory equipment in the centrifugation mode from 415 to 1660 g for 10 minutes using test tubes that do not contain separation gel. Plasma sampling for clinical use should be carried out from the middle layer of the obtained sample.