Effect of Fullerenol C60 on Erythrocyte Deformability During Ischaemia-Reperfusion Injury of Lower Extremity in Diabetic Rats

• Published in: Gazi tıp dergisi Vol. 30; no. 4; pp. 374 - 377
• Main Authors: Kip,Gülay, Kartal,Hakan, Comu,Faruk Metin, Polat,Yücel, Arslan,Mustafa, Küçük,Ayşegül
• Format: Journal Article
• Language: English
• Published: Gazi Üniversitesi Yayınları 01-01-2019
• Subjects: Sağlık Hizmetleri; Tıp; Ankara; Türkiye; ischemia reperfusion; Eritrosit deformabilitesi; rat; Erythrocyte deformability; iskemi reperfüzyon; fullerenol C60
• ISSN: 2147-2092; 2147-2092
• DOI: 10.12996/gmj.2019.96

Background: Fullerenol, a water-soluble C60-fullerene derivative synthesized
by Chiang et al, has been demonstrated to be able to scavenge free radicals
in vitro and in vivo. Although its protective effects have been already studied
and shown in ischemia reperfusion (IR) injury, additional investigation is
necessary for its effect on erythrocyte deformability. The purpose of our
study was to look into the effects of fullerenol C60 on erythrocyte
deformability in rat lower extremity ischemia reperfusion injury model.
Materials and Methods: After approval of the Ethics Committee, 30 Wistar
Albino rat were divided into 5 groups (n:6) as; Control (C), Diabetes (group
D), diabetes+ fullerenol C60 group (DF), diabetes+ IR (group DIR) and
diabetes IR+ fullerenol C60 (DIRF). 55 mg/kg streptozotocin was
administered to the rats for diabetes. After the period of 72 hour, blood
glucose concentration was mesured, 250 mg/dl and above were considered
as diabetic rat. Four week after the formation of diabetes, rats were
subjected to 2 hour ischemia and 2 hour reperfusion. Erythrocyte packs
were prepared from heparinized blood samples and deformability
measurements were performed.
Results: The deformability index was significantly increased in diabetic rats;
however, it was similar in group D, DF and DIRF. It was significantly increased
in group DIR when compared to group C, D, DF and DIRF. The relative
resistance was increased in I/R models.
Conclusion: This study aimed to investigate the effects of IR on erythrocyte
deformability which may lead to disturbance in blood flow and hence tissue
perfusion in infrarenal rat aorta. We found that fullerenol C60 had beneficial
effects by reversing undesirable effects of IR. In our opinion, further studies
with larger volume are required to support our promising results.