Efficiency of Thrips tabaci and Frankliniella occidentalis populations in transmission of Tomato yellow ring virus

• Published in: Zoology and ecology Vol. 25; no. 3; pp. 241 - 246
• Main Authors: Mortazavi, Nasimeh, Aleosfoor, Maryam
• Format: Journal Article
• Language: English
• Published: Taylor & Francis 03-07-2015
• Subjects: susceptibility of plants; thrips colonies; TYRV; vector competency; virus acquisition
• ISSN: 2165-8005; 2165-8013
• DOI: 10.1080/21658005.2015.1044165

The Tomato yellow ring virus (TYRV, family Bunyaviridae, genus Tospovirus) was found for the first time in greenhouses of Shiraz in the southern province of Fars, Iran, in 2007. TYRV, like other tospoviruses, is transmitted by thrips. Many factors affect the transmission efficiency of thrips-borne viruses such as thrips species or belonging to different populations. In this study, we evaluated the transmission efficiency of TYRV by different populations of two thrips species, Frankliniella occidentalis (Pergande) and Thrips tabaci (Lindeman) on different host plants. Transmission experiments were made setting newly hatched larvae (≤4 h) for acquisition on systemically infected leaves of Petunia hybrida, Nicotiana tabacum and Lycopersicon esculentum. The capacity of thrips to transmit the virus was assessed by use of the petunia leaf disc technique. Results by the double antibody sandwich enzyme-linked immunosorbent assay showed that different populations of both species could transmit this isolate to an indicator plant but in different efficiency. Compared to F. occidentalis, T. tabaci populations had a higher efficiency of TYRV transmission. Among the studied plants, tomato was the most susceptible in terms of virus infection. It is worth mentioning that the virus was more efficiently acquired from tomato by thrips larval stage.
Bunyaviridae šeimos Tospovirus genčiai priklausantis pomidorų geltonojo žiediškumo virusas (Tomato yellow ring virus, TYRV), Širazo (Farso provincijos Irane pietinė dalis) šiltnamiuose pirmą kartą buvo aptiktas 2007 m. Kaip ir kitus tospovirusus, jį platina tripsai. Tripsų platinamų virusų pernešimo efektyvumas priklauso nuo daugelio veiksnių, pvz., tripso rūšies arba populiacijos. Tirtas viruso pernešimo efektyvumas Frankliniella occidentalis (Pergande) ir Thrips tabaci (Lindeman) populiacijose ir skirtinguose augaluose šeimininkuose. Tyrimai buvo atliekami uždedant ką tik išsiritusias (≤4 valandų) tripsų lervas ant pažeistų Petunia hybrida, Nicotiana tabacum ir Lycopersicon esculentum lapų. Tripsų gebėjimas pernešti virusą buvo nustatomas petunijos lapo disko (petunia leaf disc technique) metodu. DAS-ELISA analizės rezultatai parodė, kad skirtingos abiejų tripsų rūšių populiacijos perneša šį izoliatą indikatoriniam augalui nevienodai efektyviai. T. tabaci virusą pernešė efektyviau, negu F. occidentalis. Nustatyta, kad pomidoras jautriausias TYRV infekcijai, ir būtent iš pomidorų tripso lervos šį virusą gaudavo dažniausiai.