Kinetics of CXCR4 and CCR5 up-regulation and human immunodeficiency virus expansion after antigenic stimulation of primary CD4+ T lymphocytes

The chemokine receptors CCR5 and CXCR4 are coreceptors for the human immunodeficiency virus (HIV) and determine the cell tropism of different HIV strains. Previous studies on their regulation were performed under conditions of unspecific T-lymphocyte stimulation and provided conflicting results. To...

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Bibliographic Details
Published in:Blood Vol. 96; no. 5; pp. 1853 - 1856
Main Authors: Maier, Reinhard, Bartolomé-Rodrı́guez, Marı́a Matilde, Moulon, Corinne, Weltzien, Hans Ulrich, Meyerhans, Andreas
Format: Journal Article
Language:English
Published: Washington, DC Elsevier Inc 01-09-2000
The Americain Society of Hematology
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Summary:The chemokine receptors CCR5 and CXCR4 are coreceptors for the human immunodeficiency virus (HIV) and determine the cell tropism of different HIV strains. Previous studies on their regulation were performed under conditions of unspecific T-lymphocyte stimulation and provided conflicting results. To mimick physiologic conditions, highly purified primary Staphylococcusenterotoxin B (SEB)-reactive CD4 T lymphocytes were stimulated in the presence of autologous antigen-presenting cells and the kinetics of CCR5 and CXCR4 surface expression and HIV replication were studied. Both chemokine receptors were transiently up-regulated with maximal expression at day 3 after stimulation. The stimulated T cells were equally susceptible to productive infection with R5-and X4-tropic virus strains. Thus, antigenic stimulation of T cells promotes efficient replication of both, T cell-tropic and macrophage-tropic HIV.
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ISSN:0006-4971
1528-0020
DOI:10.1182/blood.V96.5.1853