Molecular cloning of PCR products

It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improve...

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Bibliographic Details
Published in:	Current protocols in molecular biology (Print) Vol. Chapter 15; p. Unit 15.4
Main Authors:	Finney, M, Nisson, P E, Rashtchian, A
Format:	Journal Article
Language:	English
Published:	United States 01-11-2001
Subjects:	Base Pairing Cloning, Molecular - methods DNA - genetics DNA - isolation & purification Genetic Vectors - genetics Polymerase Chain Reaction - methods Uracil-DNA Glycosidase
Online Access:	Get more information
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Summary:	It is often desirable to clone PCR products to establish a permanent source of cloned DNA for hybridization studies, to obtain high-quality DNA sequencing results, or to separate products when PCR amplification yields a complex mixture. The efficiency of direct cloning of PCR products can be improved by generating suitable ends on the amplified fragments. This unit describes the strategies for generating and manipulating suitable ends on the PCR fragments.
ISSN:	1934-3647
DOI:	10.1002/0471142727.mb1504s56