Rapd-Analysis of Flax Varieties of the Ukrainian National Collection

• Published in: Ecological Engineering & Environmental Technology Vol. 23; no. 3; pp. 1 - 6
• Main Authors: Ihor Kovalenko, Ihor Vereshchahin, Yevheniia Butenko, Natalia Kandyba, Viktor Onychko, Olha Bakumenko, Vladyslav Kovalenko, Tetiana Klochkova
• Format: Journal Article
• Language: English
• Published: Polish Society of Ecological Engineering (PTIE) 01-05-2022
• Subjects: cluster; linum usitatissimum l; locus; primer; rapd analysis; variety sample
• ISSN: 2719-7050; 2719-7050
• DOI: 10.12912/27197050/146384

The article presents the results of research on the use of RAPD (Random Amplified Polymorphic DNA) molecular primers, linked to the sign of fiber content in the stems of flax. The main purpose of the research is to identify genetic polymorphism based on the fiber content, inter- and intraspecific genetic relationships between domestic and foreign flax varieties and hybrids. Realization of this aim will allow the number of tasks: 1) to increase the possibilities for successfully identify flax varieties and hybrids, 2) group the breeding material according to its genetic affinity, which will optimize the selection of pairs for crossing, 3) it is better to integrate traditional breeding methods (hybridization, selection, mutagenesis) with molecular biology methods for creation flax varieties with specified parameters of valuable economic traits and, as a consequence, to make selection most successful. 24 varieties and hybrids of flax were investigated using the method of polymerase chain reaction and separation of DNA fragments by gel electrophoresis. A small number of loci on electrophoregrams were detected, which indicates a small affinity of the selection material, which was also confirmed by the use of cluster analysis.