Solid-phase extraction of gallic acid and ascorbic acid in fruits using polymerized deep eutectic solvent-modified substrate

• Published in: Bioanalysis Vol. 15; no. 20; pp. 1221 - 1233
• Main Authors: Moghaddam, Ali Zeraatkar, Arabi, Elahe, Shakourian-Fard, Mehdi
• Format: Journal Article
• Language: English
• Published: Newlands Press Ltd 01-10-2023
• Subjects: ascorbic acid; dispersive solid-phase extraction; gallic acid; magnetic nanoparticles; polymerized deep eutectic solvent
• ISSN: 1757-6180; 1757-6199
• DOI: 10.4155/bio-2023-0093

Novel substrates were synthesized by porous and nonporous polymerization of deep eutectic solvents on magnetic silica nanoparticles and introduced for dispersive solid-phase extraction of two analytes.
The prepared substrates were characterized, and an extraction procedure was implemented to select the best substrates and eluent. The central composite design acted to optimize the effects of parameters that influenced the extraction efficiencies.
For gallic and ascorbic acids, the limits of detection were obtained at 0.136 and 0.165 μM, respectively, with linear ranges of 0.6–125.2 and 0.5–106.8 μM, respectively.
The substrate produced good extractions even after being used three-times and was successfully applied for the analysis of real samples.