Impaired insulin-induced platelet antiaggregating effect in obesity and in obese NIDDM patients

• Published in: Diabetes (New York, N.Y.) Vol. 44; no. 11; pp. 1318 - 1322
• Main Authors: Trovati, M., Mularoni, E. M., Burzacca, S., Ponziani, M. C., Massucco, P., Mattiello, L., Piretto, V., Cavalot, F., Anfossi, G.
• Format: Journal Article
• Language: English
• Published: American Diabetes Association 01-11-1995
• ISSN: 0012-1797; 1939-327X; 0012-1797
• DOI: 10.2337/diabetes.44.11.1318

Impaired insulin-induced platelet antiaggregating effect in obesity and in obese NIDDM patients.
M Trovati ,
E M Mularoni ,
S Burzacca ,
M C Ponziani ,
P Massucco ,
L Mattiello ,
V Piretto ,
F Cavalot and
G Anfossi
Department of Clinical and Biological Sciences, University of Turin, San Luigi Gonzaga Hospital, Orbassano, Italy.
Abstract
To investigate the effects of insulin on platelets in obesity and in non-insulin-dependent diabetes mellitus (NIDDM)--classic
insulin-resistant states--we determined ADP-induced platelet aggregation and platelet cGMP (guanosine 3',5'-cyclic monophosphate)
content in platelet-rich plasma obtained from nine obese subjects and nine age-matched healthy volunteers and from eight NIDDM
obese patients and nine age-matched healthy volunteers after a 3-min incubation with human recombinant insulin (0, 240, 480,
960, and 1,920 pmol/l). Platelet aggregation was evaluated using different ADP doses to measure the ADP concentration determined
on the basis of a dose-response curve necessary to elicit a maximal aggregation of 50% (ED50). Insulin induced a dose-dependent
decrease of platelet aggregation to ADP (P = 0.0001) in healthy subjects. A significant effect was evident starting from an
insulin concentration of 240 pmol/l. On the contrary, in insulin-resistant subjects, insulin reduced platelet sensitivity
to ADP only at a concentration of 1,920 pmol/l. When ADP ED50 values obtained in platelet-rich plasma incubated with insulin
were expressed in percentage of the ADP ED50 values obtained in platelet-rich plasma without insulin, considered as 100%,
we observed that ADP ED50 with 1,920 pmol/l insulin was 153.6 +/- 13.2% in the younger healthy subject group (P = 0.004),
150.0 +/- 3.8% in the older healthy subject group (P = 0.0001), 116.1 +/- 6.1% in obese subjects (P = 0.031), and 120.0 +/-
8.6% in NIDDM patients (P = 0.05). In healthy subjects, insulin induced a dose-dependent increase of platelet cGMP (P = 0.0001).