Interference of Ascorbic Acid with the Starch-lodine Reaction
Starch estimation in various plant tissues is accomplished using a reaction between starch and iodine. We report that the starch-iodine reaction is strongly inhibited by ascorbic acid, a normal cellular constituent of higher plants. The presence of 10−4 M ascorbic acid in the reaction mixture caused...
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| Published in: | Annals of botany Vol. 65; no. 3; pp. 281 - 283 |
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| Main Authors: | , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Oxford University Press
01-03-1990
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| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Starch estimation in various plant tissues is accomplished using a reaction between starch and iodine. We report that the starch-iodine reaction is strongly inhibited by ascorbic acid, a normal cellular constituent of higher plants. The presence of 10−4 M ascorbic acid in the reaction mixture caused an almost complete inhibition of the starch-iodine reaction. This inhibition may be alleviated by increasing the amounts of iodine-potassium iodide (IKI) in the reaction mixture, suggesting the possibility that a complex is formed between ascorbic acid and iodine. Evidence for this comes from a shift in absorption maxima of ascorbic acid due to addition of IKI. |
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| Bibliography: | istex:8964B0ABAE249F9AE86A3CF81F7A678B4BA0A9AD For correspondence. ark:/67375/HXZ-XMSWXG77-7 ArticleID:65.3.281 |
| ISSN: | 0305-7364 1095-8290 |
| DOI: | 10.1093/oxfordjournals.aob.a087935 |