Analysis of the probiotic activity of Bacillus velezensis RT-26 strain isolated from reindeer rumen by whole-genome sequencing

• Published in: BIO Web of Conferences Vol. 48; p. 3002
• Main Authors: Ilina, L.A., Filippova, V.A., Ponomareva, E.S., Brazhnik, E.A., Dunyashev, T.P., Laishev, K.A.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Les Ulis EDP Sciences 01-01-2022
• Subjects: Amino acids; Antibiotics; Bacillus velezensis; Biodegradation; Carbohydrate metabolism; Carbohydrates; Degradation; Fatty acids; Gene sequencing; Genes; Genomes; Glycolysis; Glycosidases; Glycosyl hydrolase; Hydrolase; Metabolic pathways; Metabolism; Mycotoxins; Pentose; Pentose phosphate pathway; Probiotics; Riboflavin; Rumen; Synthesis; Thiamine; Tricarboxylic acid cycle; Vitamins; Whole genome sequencing; Xenobiotics; β Lactamase
• ISSN: 2117-4458; 2273-1709; 2117-4458
• DOI: 10.1051/bioconf/20224803002

The paper analyses the properties of Bacillus velezensis RT-26, a probiotic strain isolated from reindeer rumen, which has high activity towards fiber degradation, against bacterial and fungal pathogens. The analysis was performed using whole-genome sequencing of the strain using the Illumina platform. The study revealed that strain RT-26 possessed a complete set of metabolic pathways, including glycolysis, the tricarboxylic acid cycle, and the pentose phosphate pathway. 411 genes were involved in carbohydrate metabolism in the strain genome, 229 genes were related to vitamin and coenzyme metabolism, 149 genes were involved in fatty acid metabolism. The synthesis pathways of various amino acids, most B vitamins (thiamine, riboflavin, nicotiamide, vitamin B5) were identified in the genome. A complete pathway for synthesis of the dipeptide antibiotic bacilisin was detected in the strain. In addition, the strain is capable of synthesizing class A beta-lactamase. No genes responsible for the degradation of mycotoxins and xenobiotics were detected in the genome of the strain studied. A number of glycosyl hydrolase families were detected in the strain genome: GH 1, 3, 4, 5, 6, 11, 13, 16, 18, 20, 23, 26, 28, 30, 32, 43, 46, 51, 53, 68, 68, 73, 101, 109, 126. Carbohydrate-binding proteins were of the SVM 50 family. Glycosyltransferases were of GT 1, 2, 4, 8, 26, 28, 30, 51, 83 families. In the genome of Bacillus velezensis strain RT-26, cellulases related to families GH 5, 6, 26, 51, chitinases related to families GH 18 and 23, and xylanases related to families GH 1, 3, 4, 16, 30, 43 were found. Thus, strain B. velezensis RT-26 has several phenotypically and genotypically proven properties that can characterize it as a good probiotic microorganism.