An approach to fast 2D nuclear magnetic resonance at low concentration based on p-H 2 -induced polarization and nonuniform sampling

• Published in: Magnetic resonance in chemistry Vol. 59; no. 12; pp. 1236 - 1243
• Main Authors: Aspers, Ruud L E G, Tessari, Marco
• Format: Journal Article
• Language: English
• Published: England 01-12-2021
• Subjects: Hydrogen; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; NMR; nonuniform sampling; SABRE; PHIP; hyperpolarization; Para-hydrogen; SMILE
• ISSN: 0749-1581; 1097-458X
• DOI: 10.1002/mrc.5182

Recent developments in para-hydrogen-induced polarization (PHIP) methods allow the nuclear magnetic resonance (NMR) detection of specific classes of compounds, down to sub-micromolar concentration in solution. However, when dealing with complex mixtures, signal resolution requires the acquisition of 2D PHIP-NMR spectra, which often results in long experimental times. This strongly limits the applicability of these 2D PHIP-NMR techniques in areas in which high-throughput analysis is required. Here, we present a combination of fast acquisition and nonuniform sampling that can afford a 10-fold reduction in measuring time without compromising the spectral quality. This approach was tested on a mixture of substrates at micromolar concentration, for which a resolved 2D PHIP spectrum was acquired in less than 3 min.