Secuencia parcial del genoma del maxicírculo de Leishmania braziliensis, comparación con otros tripanosomátidos

• Published in: Universitas scientiarum Vol. 16; no. 1; pp. 29 - 50
• Main Authors: Nocua, Paola, Ramírez, Cesar, Requena, José María, Puerta, Concepción Judith
• Format: Journal Article
• Language: English
• Published: Pontificia Universidad Javeriana 01-04-2011
• Subjects: kinetoplast DNA (kDNA); Leishmania (Viannia) braziliensis; maxicircle; RNA editing
• ISSN: 0122-7483; 2027-1352
• DOI: 10.11144/javeriana.SC16-1.mgps

Objective. With the aim to provide new insights for genotyping and phylogenetic studies of the Leishmania genus, in this study the sequence of the maxicircle in Leishmania braziliensis, strain MHOM-BR-75-M2904, was determined and compared with those reported in other trypanosomatids species. Materials and methods. Searches for maxicircle sequences were performed in the unassembled sequences of GeneDB database version 2.1, as well as in the GenBank, using the ND8 and RPS12 genes of L. braziliensis as the initial probes. These sequences were assembled and compared with the homologous sequences of trypanosomatids using the bioinformatics tools LALIGN and ClustalW2. The size of maxicircle was determined by Southern blot assays. Results. Two maxicircle fragments of 6535 and 4257 nucleotides were assembled. The sequences of these genes showed high synteny and similarity with the sequences in other Leishmania species. This similarity even was extended to the editing patterns of these molecules. Conclusions. Although L. braziliensis is the most divergent species of the Leishmania genus in their nuclear genome, the maxicicircle has a high conservation. This result suggests that the pattern of editing present in the different Leishmania species studied has been conserved also in the subgenus Viannia. These results indicate a high conservation in the editing of mitochondrial transcripts at the genus level. Key words: kinetoplast DNA (kDNA), RNA editing, Leishmania (Viannia) braziliensis, maxicircle.